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Video file corresponding to Figure 1A.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows discrete biofilms containing live bacteria alongside host cells that are morphologically suggestive of immune cells. Scale 10 μm.
YouTube URL: https://youtu.be/fsQiSyCijfg
Video file corresponding to Figure 1B.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with bronchiectasis that shows discrete biofilms containing live bacteria alongside host cells that are morphologically suggestive of immune cells. Scale 10 μm.
YouTube URL: https://youtu.be/eean7E0_JsU
Video file corresponding to Figure 1C.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows large biofilms containing both live and dead bacteria embedded within an extensive extracellular DNA structure. Scale 10 μm.
YouTube URL: https://youtu.be/H5e-LqDJwMA
Video file corresponding to Figure 1D.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with bronchiectasis that shows large, discrete biofilms containing live and dead bacteria. This image includes intact host cells that are morphologically consistent with macrophages and lymphocytes. Scale 10 μm.
YouTube URL: https://youtu.be/lQlqxKYQ6IU
Video file corresponding to Figure 1E.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows large biofilms containing live and dead bacteria observed in close association with extracellular DNA and immune cells. Scale 10 μm.
YouTube URL: https://youtu.be/8AWwHQyn9Pc
Video file corresponding to Figure 1F.
The video demonstrates optical sectioning through a BacLight-stained bronchoalveolar lavage from a child with bronchiectasis that shows an extensive non-squamous epithelial cell biofilm and microcolonies containing live and dead bacteria embedded within an extensive extracellular DNA matrix together with immune cells. Scale 10 μm.
YouTube URL: https://youtu.be/R1Q9gkF8UQg
Video file corresponding to Appendix Figure 1A.
The video demonstrates optical sectioning through a lectin-stained bronchoalveolar lavage showing bacteria (orange) and extracellular matrix (green) within a non-squamous epithelial cell biofilm. Nuclei of the host cells are also stained orange. Scale 10 μm.
YouTube URL: https://youtu.be/m90tnxy3WAQ
Video file corresponding to Appendix Figure 1B.
The video demonstrates optical sectioning through a lectin-stained bronchoalveolar lavage showing bacteria (orange) and extracellular matrix (green) within a squamous epithelial cell biofilm. Nuclei of the squamous cell are also stained orange. Scale 10 μm.
YouTube URL: https://youtu.be/sjQoRlfSGUY
Video file corresponding to Appendix Figure 2A.
The video demonstrates optical sectioning through a large non-squamous epithelial cell biofilm embedded within an actively produced extensive neutrophil extracellular trap structure, as evidenced by positive neutrophil elastase stain (red) overlaid on DNA (yellow) with a negative actin stain (blue). Scale 10 μm.
YouTube URL: https://youtu.be/QU53Ekgyk0Y
Video file corresponding to Appendix Figure 2B.
The video demonstrates optical sectioning through a non-squamous epithelial cell biofilm observed in close association with intact host cells, necrotic neutrophils and extracellular DNA. Necrotic neutrophils are evidenced by positive neutrophil elastase stain (red) overlaid on DNA (yellow) and actin stains (blue). Scale 10 μm.
YouTube URL: https://youtu.be/9dRvNjVjvFw
Video file corresponding to Figure 1G.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows a large polymicrobial non-squamous epithelial cell biofilm that is positive for Moraxella catarrhalis (red) and other unidentified bacteria (Eubacterial probe, yellow) associated with host DNA and non-hybridised bacteria (blue). Scale 10 μm.
YouTube URL: https://youtu.be/8AitU5Pesl0
Video file corresponding to Figure 1H.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage from a child with bronchiectasis that shows a large polymicrobial non-squamous epithelial cell biofilm that is positive for Streptococcus pneumoniae (green) and other unidentified bacteria (Eubacterial probe, yellow) associated with host DNA and non-hybridised bacteria (blue); planktonic Haemophilus influenzae cells (red) are also evident in this image. Scale 10 μm.
YouTube URL: https://youtu.be/Rcb2rBcBo4M
Video file corresponding to Figure 2A.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows large biofilms containing live and dead bacteria attached to squamous epithelial cells. Scale 10 μm.
YouTube URL: https://youtu.be/vodJSRduLKk
Video file corresponding to Figure 2B.
The video demonstrates optical sectioning through a BacLight stained bronchoalveolar lavage from a child with bronchiectasis that shows biofilm attached to a squamous epithelial cell. Scale 10 μm.
YouTube URL: https://youtu.be/pPqKzGlLu3A
Video file corresponding to Figure 2C.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage from a child with protracted bacterial bronchitis that shows a squamous epithelial cell biofilm that contains small clusters of Moraxella catarrhalis (red) and other unidentified bacteria (Eubacterial probe, yellow) associated with host DNA (blue). Scale 10 μm.
YouTube URL: https://youtu.be/F-Wo_fNL260
Video file corresponding to Figure 2D.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage from a child with bronchiectasis that shows a squamous epithelial cell biofilm positive for Streptococcus pneumoniae (green) and other unidentified bacteria (Eubacterial probe, yellow) associated with host DNA (blue). The circle indicates the associated squamous epithelial cell. Scale 10 μm.
YouTube URL: https://youtu.be/ySK-tzhNMZA
Video file corresponding to Appendix Figure 3A.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage that shows Prevotella spp (green) and other unidentified bacteria (Eubacterial probe, yellow) present as planktonic bacterial cells associated with host DNA. Scale 10 μm.
YouTube URL: https://youtu.be/rZPpnuL3kDg
Video file corresponding to Appendix Figure 3B.
The video demonstrates optical sectioning through a fluorescent in situ hybridisation-stained bronchoalveolar lavage that shows a large squamous epithelial cell biofilm positive for the Prevotella spp probe (green) overlaid on signal from the Eubacterial probe (yellow) consistent with a monospecies biofilm (no other bacteria were observed in this biofilm). Scale 10 μm.
YouTube URL: https://youtu.be/mOCalCfwRC0
DOI: https://doi.org/10.1016/S2666-5247(21)00300-1
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